Table 1.
Summary of Four Immunohistochemical Methods Utilized in the Study.
| Antibody | Vendor | HIER (min) | Buffer | Dilution | Detection | Chromogen | Autostainer |
|---|---|---|---|---|---|---|---|
| SP142 | Spring Bioscience | 35 | DaVinci (BioCare, Concord, CA) | 1:100 | Polymer | DAB | Dako |
| E1L3N | Cell Signaling Technology | 35 | DaVinci (BioCare) | 1:200 | Polymer | DAB | Dako |
| RBT-PDL1 | Bio SB | 30 | Tris-EDTA (Thermo Fisher, Waltham, MA) | 1:50 | Polymer | DAB | Dako |
| 28-8a | Abcam | 20 | Unknown | Unknown | Polymer | DAB | Dako |
Abbreviation: HIER, heat-induced epitope retrieval.
a
28-8 Assay was performed by Bristol-Myers Squibb Canada (BMS) according to the manufacturer’s instruction using previously validated protocol.19