Effects of PGE on body fat mass, energy expenditure, and mRNA expression of adipogenic and thermogenic genes via regulation of fatty acid oxidation in epididymal white adipose tissue in HFD-fed C57BL/6J mice. The data are the mean ± SEM (n = 10). (A) The weight of adipose tissue; (B) representative photographs of adipocytes in the epididymal WAT of the mice, 200× magnification; and (C) energy expenditure and oxygen consumption (Vo2). Adipogenesis (D); thermogenesis (E); and adipokine (F) related gene expression. (G) Western blot analysis of β-actin, PPARγ, CD36, PGC1α, and CPT2 expression. ND, mice fed a normal diet; HFD, mice fed a high-fat diet (HFD) alone; PGE, Platycodon grandiflorus root extract (5%, w/w)-treated HFD-fed mice; WAT, white adipose tissue.