Table 2. Imprecise NHEJ frequencies per transfected cell.

Experiment*	NHEJ frequency
Untransfected	<1.4 × 10-4 (0)†
1	1.2 × 10-3 (10)
2	1.6 × 10-3 (13)
Mean	1.4 × 10-3 (23)

^*I-SceI expression and 1165A/DR1-13 plasmids (50 and 10 μg, respectively) were electroporated into 4 × 10⁶ LMH3.2 cells. After 3 days in culture, genomic DNA was purified and assayed for imprecise NHEJ at the target cleavage sites as described in Methods.

^†Number of NHEJ products observed.