Table 3. Frequency of left and right viral-cell junctions per transfected cell.

Experiment*	Frequency of left junctions	Frequency of right junctions
Control†	<1.1 × 10-6 (0)‡	<6 × 10-6 (0)
1	7.8 × 10-5 (27)	4.6 × 10-5 (8)
2	1.2 × 10-4 (41)	ND

ND, not determined.

^*I-SceI expression and 1165A/DR1-13 plasmids (50 and 10 μg, respectively) were electroporated into 4 × 10⁶ LMH 3.2 cells. After 3 days in culture, genomic DNA was purified and assayed for viral-cell junctions at the target cleavage sites as described in Methods.

^†Control transfections electroporated with DHBV vector alone.

^‡Number of viral-cell junctions observed.