Table 5. Sequence distribution of integrated wild-type 1165A and mutant 1165A/DR1-13 DHBV.

Experiment*	No. analyzed	Wild type, %	Mutant, %
1	8	0	100
2	37	0	100
Total	45	0	100

^*I-SceI expression and a 1:1 mixture of 1165A and 1165A/DR1-13 plasmids (50 and 10 μg, respectively) were electroporated into 4 × 10⁶ LMH3.2 cells. After 3 days in culture, genomic DNA was purified and assayed for viral-cell junctions at the target cleavage sites as described in Methods. Genotypes were determined by direct sequencing of the PCR products.