Table 1. Summary of the redshift to heme caused by Aβ and the effect of zinc and copper.
Heme-b, 50 μM
|
Aβ(1-40) (50 μM) added relative to metals
|
Zn2+, 100 μM
|
Cu2+, 100 μM
|
Redshift in heme spectrum
|
|
---|---|---|---|---|---|
Before | After | ||||
+ | + | - | - | - | 20 nm |
+ | + | - | + | - | 20 nm |
+ | + | - | - | + | 20 nm |
+ | + | - | + | + | 20 nm |
+ | - | + | + | - | Weak |
+ | - | + | - | + | Weak |
+ | - | + | + | + | Weak |
+, reagent included in the mixture. -, reagent was not included in the mixture. Zn2+, ZnCl2; Cu2+, CuSO4.