Table 1. Body composition and metabolite measurements in G86R mice.
| 75 days old (asymptomatic)
|
105 days old (early symptomatic)
|
|||
|---|---|---|---|---|
| WT | G86R | WT | G86R | |
| Body weight, g | 27.6 (24.3-31.3, 19) | 24.1 (20.3-28.0, 20)* | 31.8 (27.7-38.0, 36) | 21.5 (15.6-28.2, 34)*# |
| WAT, % | 2.14 (1.23-3.28, 19) | 1.24 (0.30-2.64, 20)* | 2.95 (0.92-6.16, 36) | 1.21 (0.01-4.43, 34)* |
| Retroperitoneal WAT, g | 0.23 (0.08-0.48, 19) | 0.10 (0.00-0.34, 20)* | 0.40 (0.10-1.16, 36) | 0.10 (0.00-0.44, 34)* |
| Epididymal WAT, g | 0.37 (0.24-0.56, 19) | 0.20 (0.06-0.40, 20)* | 0.56 (0.20-1.18 36) | 0.19 (0.00-0.64, 34)* |
| Liver, % | 5.00 (1.20-1.60, 19) | 5.30 (0.96-1.56, 20) | 4.80 (1.24-1.80, 36) | 4.70 (0.60-1.52, 36) |
| Water, % | 64.6 (14.5-19.0, 11) | 65.8 (13.1-16.4, 11) | 59.4 (15.8-19.7, 13) | 64.8 (9.4-16.3, 14,)* |
| Proteins, % | 20.9 (4.63-5.97, 11) | 20.7 (3.98-5.27, 11) | 19.9 (5.40-6.44, 13) | 22.7 (3.15-5.48, 14)*# |
| Lipids, g | 11.6 (2.04-4.18, 11) | 10.6 (1.74-3.30, 11) | 17.4 (2.03-10.1, 13) | 8.84 (0.62-3.33, 14)* |
| Minerals, g | 3.49 (0.85-1.04, 11) | 3.31 (0.69-0.89, 11) | 3.30 (0.92-1.23, 13) | 3.99 (0.62-1.17, 14)*# |
| Leptin, ng/ml | 2.63 (1.03-4.85, 7) | 1.62 (0.51-3.59, 7)* | 3.06 (0.86-8.14, 7) | 1.27 (0.00-4.75, 7)* |
| Insulin, ng/ml | ND | ND | 3.36 (2.39-4.33, 3) | 0.87 (0.45-1.35, 3)* |
| Corticosterone, ng/ml | 71 (25-123, 7) | 118 (32-293, 7) | 77 (25-165, 7) | 364 (92-754, 7)*# |
| Free T3, ng/ml | 1.18 (0.80-1.79, 11) | 1.01 (0.68-1.58, 11) | 1.69 (1.18-4.77, 11) | 0.63 (0.27-1.04, 11)* |
| β-Hydroxybutyrate, μM | 370 (13-713, 7) | 419 (65-737, 7) | 333 (32-694, 7) | 931 (51-3720, 7)*# |
| Free fatty acids, mM | 0.87 (0.42-1.84, 13) | 0.89 (0.52-1.45, 13) | 0.98 (0.43-1.42, 13) | 0.96 (0.44-1.77, 13) |
| Glucose, fed, mg/l | 195 (141-229, 25) | 182 (138-217, 25) | 143 (118-177, 12) | 116 (52-161, 13)*# |
| Glucose, fasted, mg/l | 80 (62-142, 30) | 101 (64-135, 30)* | ND | ND |
| Ob(leptin) mRNA, a.u.† | 1.00 (0.71-1.30, 4) | 0.85 (0.26-1.12, 4) | 1.00 (0.53-1.04, 4) | 0.50 (0.24-0.73, 4)* |
WAT is expressed as percentage of body weight. Data are means; values in parentheses are (i) the range of variability expressed either as in the same units as the corresponding parameter (e.g., body weight) or as grams for those measurements in percentage (e.g., WAT), and (ii) the number of animals. ND, nondetermined. *, P < 0.05, vs. corresponding WT; #, P < 0.05, vs. 75-day-old G86R mice.
†
mRNA levels are normalized relative to the corresponding WT littermates.