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. Author manuscript; available in PMC: 2016 Sep 27.
Published in final edited form as: J Allergy Clin Immunol. 2015 May 14;136(2):476–9.e6. doi: 10.1016/j.jaci.2015.02.040

Figure 1.

Figure 1

Semi-quantitative PCR and quantitative real-time PCR results of Minigene expression. A) Minigene 1 (IVS1+1G>T) mRNA expression, B) quantitative Taqman results showing only 16% expression of the wild type mRNA in Minigene-1, C) Minigene-5 (IVS5+2T>A) mRNA expression, and D) Sanger sequencing confirmation of IVS5+2T>A resulting in exon 5 skipping.