Figure 2. The accumulation of viral proteins and RNAs is reduced in DDX19-depleted cells.

A549 cells were treated with control (C) or DDX19 (19) siRNAs and infected with WSN (5 pfu/cell). (a) Total extracts were prepared at the indicated times post-infection and analyzed by immunoblots using antibodies directed against the indicated proteins. Results representative of 3 independent experiments are shown. Cropped blots are shown. The corresponding full-length blots are shown in Figure S3. (b–e) The levels of NP or NA mRNAs and vRNAs (b or c and d or e, respectively) were determined at the indicated times post-infection by strand specific RT-qPCR and were normalized to the level of the same RNA species at 3 hpi in cells treated with the control siRNAs. The results are expressed as the mean ± SEM of three independent experiments and the significance was tested with a one-sample t test using GraphPad Prism Software (*p < 0.05; **p < 0.01; ***p < 0.001). Dashed lines were used to indicate that the Y-axes have been segmented. Different scales were used for the mRNA and vRNA graphs.