Fig. 8.

Multiple sequence alignment of several MATE protein sequences. Sequence alignment of the reference proteins (LOC_Os01g49120 and AT1G73700) with NorM-NG, NorM-VC, NorM-VP, and hMATE1. Regions of transmembrane helices (TM) in LOC_Os01g49120 are outlined and numbered. Blue dots and triangles indicate amino acids responsible for functional divergence, positive selection and coevolution in the reference sequence AT1G73700. Red dots and triangles indicate amino acids responsible for functional divergence, positive selection, and coevolution in the reference sequence LOC_Os01g49120. The cyan and red outlined boxes represent amino acids that might coordinate cations and interact with substrates, respectively