Table 1.
Primer sequences for real-time PCR
| Target | Forward primer (5′-3′) | Reverse primer (5′-3′) | Gene ID |
|---|---|---|---|
| β-actin | TCGGTGACATCAAAGAGAAG | GATGCCACAGGATTCCATA | 11461 |
| β-OH Butyrate Dehydrogenase 1 | AGGCTGTGACTCTGGATTTGGG | CTGGATGGTTCTCAGTCGGTCA | 71911 |
| β-OH Butyrate Dehydrogenase 2 | AGGAGCTGGAAAGACCGAGG | TCGCAATCCAGGATGGTTCCGT | 69772 |
| 3-hydroxy-3-methylglutaryl-CoA Synthase II | AGAGAGCGATGCAGGAAACTT | AAGGATGCCCACATCTTTTGG | 15360 |
| Peroxisome Proliferator Activated Receptor α | AGAGCCCCATCTGTCCTCTC | ACTGGTAGTCTTGCAAAACCAAA | 19013 |
| Uncoupling Protein 2 | ATGGTTGGTTTCAAGGCCACA | CGGTATCCAGAGGGAAAGTGAT | 22228 |
| Glucose 6 Phosphatase | CGACTCGCTATCTCCAAGTGA | GTTGAACCAGTCTCCGACCA | 14377 |
| Phosphoenolpyruvate Carboxykinase | CTGCATAACGGTCTGGACTTC | CAGCAACTGCCCGTACTCC | 18534 |
| Carnitine Palmitoyltransferase I | CTCCGCCTGAGCCATGAAG | CACCAGTGATGATGCCATTCT | 12894 |
Annealing temperature for all primer pairs was 58 °C, except HMGCS2 which was 55 °C