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. 2016 Aug 24;17(9):1389. doi: 10.3390/ijms17091389

Figure 3.

Figure 3

Intracellular IGFBP3 inhibits osteogenic differentiation. (A) siRNA knockdown of IGFBP3 induced ARS staining in ASCs at 7 and 14 days (40×); (B) quantification of ARS staining was measured at 7 days; (C,D) siRNA knockdown of IGFBP3 significantly attenuated hypoxia-reduced osteogenic induction markers such as RUNX2, osteocalcin, and osterix at 7 and 14 days; (E) hypoxia increased the transfer of IGFBP3 into the nuclear region of ASCs, and IGFBP3 signal (green) is increased in the nuclear region (DAPI, blue) of ASCs. ** p < 0.01.