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. 2016 Aug 24;17(9):1389. doi: 10.3390/ijms17091389

Figure 6.

Figure 6

Hypoxia suppresses the osteogenic differentiation of clonal BM-MSCs. (A) Hypoxia (2%) induced the mRNA expression of IGFBP3 in clonal BM-MSCs; (B) hypoxia suppressed the osteogenic differentiation of clonal BM-MSCs at 7 and 14 days (40×); (C) quantification of ARS staining was measured at 7 days; (D) osteogenic induction markers such as RUNX2, osteocalcin, and osterixwere significantly reduced by hypoxia in DMEM and ODM; (E) siRNA-mediated inhibition of IGFBP3 significantly attenuated the hypoxia-suppressed osteogenic differentiation of clonal BM-MSCs at 7 and 14 days (40×); (F) quantification of ARS staining was measured at 7 days; (G,H) osteogenic induction markers such as RUNX2, osteocalcin, and osterix were significantly reduced by hypoxia at 7 days (G) and 14 days (H). Normoxia: black bars, hypoxia: white bars. * p < 0.5, ** p < 0.01.