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. 2016 Aug 24;17(9):1397. doi: 10.3390/ijms17091397

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Silence effect of CaSR small interfering RNA (siRNA) transfection. T lymphocytes were transfected with 5 μg positive plasmid or negative plasmid. Protein or mRNA was harvested 24 h post-transfection. Western blot (A) and qRT-PCR (B) results showed that the positive CaSR siRNA plasmid transfection in T lymphocytes reduced the expressions of CaSR mRNA and protein, which increased remarkably in AMI patients. In addition, negative plasmid of CaSR siRNA had no effect on the CaSR expression. * p < 0.05 vs. normal group; ^Δ p < 0.05 vs. AMI group.

Silence effect of CaSR small interfering RNA (siRNA) transfection. T lymphocytes were transfected with 5 μg positive plasmid or negative plasmid. Protein or mRNA was harvested 24 h post-transfection. Western blot (A) and qRT-PCR (B) results showed that the positive CaSR siRNA plasmid transfection in T lymphocytes reduced the expressions of CaSR mRNA and protein, which increased remarkably in AMI patients. In addition, negative plasmid of CaSR siRNA had no effect on the CaSR expression. * p < 0.05 vs. normal group; ^Δ p < 0.05 vs. AMI group.