Figure 5.

Measurement of IL-6 production from hardwood (A) and softwood (B) smoke exposure and RV-16 infection. Primary human lung fibroblasts (n = 4) were stimulated with hardwood and softwood smoke extract at 0.1% and 1% concentration alone, RV-16 infection alone (MOI = 1), or both and incubated for 24 h. Unstimulated fibroblasts were also measured for IL-6 constitutive release. Supernatants were collected for IL-6 concentration via ELISA. Data expressed as pg/mL. Statistical analysis was executed using two-way ANOVA with Sidak’s post-test.