Figure 3.

Demonstration of apoA-I NO₂Tyr and MPO enrichment within HDL-like particles isolated from human atherosclerotic lesions. Left and center: Equal amounts of protein (40 μg per lane) from either HDL-like particles isolated from human atherosclerotic lesions (n = 12 subjects, pooled) or HDL isolated from pooled plasma from healthy donors were analyzed by SDS-PAGE (10–20% gradient gels), transferred onto PVDF membranes, and probed using antibodies to either anti-NO₂Tyr (left) or anti–apoA-I (center), and then visualized by brief chemiluminescence exposure, as described in Methods. Right: Isolated human MPO standard (std) or HDL-like particles isolated from human atherosclerotic lesions (40 μg total protein, n = 12 subjects, pooled) were analyzed by SDS-PAGE (10–20% gradient gels), transferred onto PVDF membranes, probed using antibodies to human MPO, and then visualized by brief chemiluminescence exposure, as described in Methods.