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. 2016 Aug 27;17(9):1416. doi: 10.3390/ijms17091416

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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RAxML phylogenetic trees were estimated using 24 hepatitis C virus (HCV) reference sequences (black) downloaded from Los Alamos HCV Sequence Database and 8 HCV isolates (red) in this study for NS3 (A) and NS5B (B) regions, respectively. The reliability of the phylogenetic clustering was evaluated using bootstrap analysis with 1000 replicates. Bootstrap support values are only shown for the HCV1b clades (yellow area). The scale bars at the bottom of the figure represent genetic distance.