Table 1.
Summary of Experiment 2.
| Treatment | No. of Stable Transfectants | Fluorescence Expression 1 | ||
|---|---|---|---|---|
| R/G | R | G | ||
| Experimental group | ||||
| 1 | 13 | 12 | 0 | 1 |
| 2 | 10 | 10 | 0 | 0 |
| 3 | 18 | 16 | 0 | 2 |
| Control group | ||||
| 1 | 0 | – | – | – |
| 2 | 0 | – | – | – |
| 3 | 0 | – | – | – |
1 PEFs (2 × 105) were transfected in a nucleofector solution containing seven PB vectors and pTrans (Experimental group), or only seven PB vectors without pTrans (Control group). After selection with multiple selection drugs, the emerging colonies were picked and propagated as stable transfectants. They were then examined for fluorescence expression under a fluorescence microscope and classified as R/G (cells expressing both EGFP and tdTomato fluorescence), R (cells expressing tdTomato alone), and G (cells expressing EGFP alone). Transfection was performed three times.