Table 2.
Estimation of the copy number of transgenes in the PEF transfectants 1.
| Transfectants | pT-EGFP | pT-tdTomato | pT-neo | pT-pac | pT-bsr | pT-hph | pT-Sh ble |
|---|---|---|---|---|---|---|---|
| mPB-1 | 4 | 2 | 2 | 2 | 1 | 4 | 3 |
| mPB-2 | 7 | 7 | 5 | 8 | 4 | 2 | 1 |
| mPB-3 | 6 | 5 | 7 | 4 | 3 | 2 | 7 |
1 Estimation of the copy number of each transgene integrated into the host genome of PEF transfectants was performed by PCR using serially diluted transposon DNA, as described in the Materials and Methods section. Identification of each transposon was performed using the following primer sets [23]: EGFP-7S/EGFP-7RV for pT-EGFP, TDR-3S/TDR-3RV for pT-tdTomato, Ne-4S/Ne-4RV for pT-neo, puro-S/puro-3RV for pT-pac, bsr-S/bsr-RV for pT-bsr, Hyg-3S/Hyg-RV for pT-hph, and ble-F/ble-B for pT-Sh ble.