Table 1.
Descriptive statistics and phenotypic correlations between drip loss and metabotypes.
| Traits | Mean ± SD 1 | Min 2 | Max 3 | Correlation to Drip Loss 4 |
|---|---|---|---|---|
| drip loss, % | 1.97 ± 1.40 | 0.40 | 5.30 | 1 |
| pH1 | 6.53 ± 0.22 | 5.89 | 6.94 | −0.31 ** |
| pH24 | 5.52 ± 1.12 | 5.32 | 6.06 | −0.35 *** |
| PKM | 26,454.10 ± 17,829.55 | 13.47 | 88,251.64 | −0.20 * |
| PGAM2 | 5600.37 ± 4985.98 | −10.77 | 32,935.16 | −0.19 |
| FBPase | 27,407.08 ± 20,231.70 | 809.35 | 114,192.30 | −0.11 |
| TPI1 | 1754.68 ± 1526.65 | 32.13 | 7802.84 | −0.21 * |
| pyruvic acid | 4.32 × 10−2 ± 3.62 × 10−2 | 6.16 × 10−3 | 2.11 × 10−1 | 0.22 * |
| lactic acid | 6.49 × 10−1 ± 3.28 × 10−1 | 1.88 × 10−1 | 1.64 | 0.08 |
| glucose | 9.02 × 10−3 ± 1.32 × 10−2 | 1.21 × 10−4 | 8.41 × 10−2 | 0.19 |
| phosphoenol pyruvate | 5.59 × 10−2 ± 8.95 × 10−2 | 1.80 × 10−3 | 0.53 | 0.13 |
| glycerone-p | 1.86 ± 1.10 | 2.48 × 10−1 | 5.85 | 0.07 |
| DG3P | 2.56 × 10−1 ± 4.09 × 10−1 | 2.61 × 10−3 | 2.61 | 0.14 |
| fumaric acid | 2.67 × 10−3 ± 1.25 × 10−3 | 5.50 × 10−4 | 7.23 × 10−3 | 0.12 |
| succinic acid | 1.38 × 10−2 ± 5.02 × 10−3 | 3.23 × 10−3 | 3.23 × 10−2 | −0.02 |
| malic acid | 6.03 × 10−3 ± 2.92 × 10−3 | 8.85 × 10−4 | 1.64 × 10−2 | 0.11 |
| methylglyoxal | 9.62 × 10−3 ± 5.44 × 10−3 | 2.61 × 10−4 | 2.89 × 10−2 | 0.22 * |
| glycine | 8.59 × 10−2 ± 2.39 × 10−2 | 4.84 × 10−2 | 1.62 × 10−1 | 0.11 |
| hydroxypyruvic acid | 1.06 × 10−2 ± 6.81 × 10−3 | 1.76 × 10−3 | 4.98 × 10−2 | 0.02 |
| F6P | 2.17 × 10−2 ± 3.43 × 10−2 | 2.91 × 10−4 | 2.25 × 10−1 | 0.12 |
| serine | 6.04 × 10−3 ± 2.99 × 10−3 | 1.76 × 10−3 | 2.15 × 10−2 | −0.01 |
| glycerone | 1.41 × 10−1 ± 8.36 × 10−2 | 2.17 × 10−2 | 4.37 × 10−1 | 0.20 |
| ceramide | 1.68 × 10−4 ± 1.24 × 10−3 | 2.33 × 10−6 | 6.59 × 10−4 | 0.05 |
| glucosylceramide | 2.46 × 10−3 ± 4.72 × 10−3 | 1.69 × 10−4 | 2.72 × 10−2 | 0.21 * |
| phosphoethanolamine | 8.57 × 10−4 ± 5.01 × 10−4 | 2.28 × 10−4 | 3.52 × 10−3 | 0.12 |
Drip loss measured in Musculus longissimus dorsi (LD) 24 h post-mortem (p.m.); pH measured 1 h post-mortem (p.m.) (pH1) and 24 h p.m. (pH24) in LD; 1 mean and standard deviation (SD); 2 minimum (Min); 3 maximum (Max); 4 calculation of correlation coefficients based on residuals; Mean, SD, Min and Max of proteins are based on signal dependent intensities of ion fragments (in mass) relative to time, so-called selection reaction monitoring (SRM) intensities; The units of the metabolite profiles are based on mass intensities, recorded by GC-MS and LC-QTOF/MS, normalized to an internal standard; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; glycerone-p = dihydroxyacetone phosphate; PGAM2 = phosphoglycerate mutase 2 (muscle); PKM = pyruvate kinase (muscle); FBPase = fructose-1,6-bisphosphatase 2; TPI1 = triose phosphate isomerase 1; DG3P = d-glycerate-3-phosphate; F6P = fructose-6-phosphate; bold: proteins.