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. 2016 Sep 2;17(9):1427. doi: 10.3390/ijms17091427

Figure 3.

Figure 3

Effect of miR-378 on the mRNA and protein expression levels of its target gene Caspase-3. (A,B) Representative Western blot and quantitative analysis showed the caspase-3 protein levels increased significantly in N2A cells at 0–24 h of reoxygenation after 3 h of OGD, * p < 0.05, n = 5 per group; (C,D) Transfection of pri-miR-378 effectively decreased the expression of caspase-3, whereas anti-miR-378 increased the expression of caspase-3 in mouse N2A cells exposed to 3 h OGD/24 h reoxygenation, * p < 0.05 vs. OGD non-trans, n = 3 per group; (E) Upper panel, construction of luciferase reporter system; Lower panel, pri-miR-378 decreased luciferase activity of the reporter vector containing 3′-UTR of caspase-3, but had no effect on the mutated reporter vector, * p < 0.05, n = 6 per group; (F) Pri-miR-378 and anti-miR-378 had no effect on Caspase-3 mRNA levels, n = 5 per group.