Figure 6.

P. emblica (PE) treatment protected against nocodazole-induced micronuclei (MN) in NCM460 cells. NCM460 cells were either co-treated with PE and nocodazole (ND) for 6 h (I) or pre-treated with PE for 6 h (II) and 72 h (III) before a 6-h ND challenge. Cells were then allowed to recover for 1 h before cytochalasin B was added to block cytokinesis (10 h) for MN assessment. MN assessment obtained from more than 1000 binucleated cells and then were set relative to the values measured in untreated control cells, which were arbitrarily set at 1. Similar results were observed in three independent experiments. The numbers above bars indicate the percentages of reduction in ND-induced MN caused by PE from each experimental scheme calculated using the formula described in Materials and Methods. The symbols + and − represent cells were treated with or without indicated components, respectively. Error bars indicate one SEM. ** p < 0.01; *** p < 0.001 vs. untreated control. ns, not significant.