Figure 5.

Behavioral responses were compared before (pre-odor) and after (odor trial) delivery of an amino acid (A) or bile salt (B) mixture. Control fish made significantly more turns after exposure to either mixture. (A) At 2 days following zinc sulfate treatment, fish did not show a statistically significant response to amino acids; however, there appeared to be a subset of fish that showed some response to the odor (2 days) and others who exhibited no change in behavior in response to the odor (2 days’). By 10 and 14 days after chemical exposure, fish made more turns during the amino acid odor trial; (B) Two days after exposure to zinc sulfate, fish did not respond to the bile salts mixture. Even after 10 days, the response to bile salts was not different from the pre-odor behavior. However, when given 14 days following zinc sulfate exposure the ability to perceive bile salts was regained. * p < 0.05.