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. 2016 Sep 1;17(9):1451. doi: 10.3390/ijms17091451

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of primary and Phase II antioxidant and detoxifying enzyme mRNA levels in RAW264.7 cells. RAW264.7 cells were pretreated for 24 h with specified concentrations of LX519290. The mRNA expression of primary antioxidant enzyme (A); and phase II antioxidant and detoxifying enzyme (B) were measured by reverse transcription polymerase chain reaction (RT-PCR). The protein level of haeme oxygenase-1 (HO-1) in time dependent (C); and concentration dependent (D); and the nuclear translocation of NF-E2-related factor-2 (Nrf2) in time dependent (E); and concentration dependent (F) were analyzed by Western blot.