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. 2016 Sep 2;17(9):1467. doi: 10.3390/ijms17091467

Figure 2.

Figure 2

Epigenetic silencing of GATA6 through the activation of STAT3 suppresses expression of trefoil factors (TFF) 1 and 2 in gastric cancer: (A) Relative expression levels of GATA6 in immortalized gastric epithelial cells (GES) and gastric cancer cell lines as determined by RT-PCR; (B) Methylation level of the GATA6 promoter in gastric cancer cell lines, as determined by bisulfite pyrosequencing. Relative expression levels of (C) TFF1 and (D) TFF2 in the same gastric cancer cell lines, as determined by RT-PCR; (E) Western blot analysis showing the protein levels of STAT3 and phosphorylated STAT3 (pSTAT3) in AGS gastric cancer cells treated with various quantities of STAT3 inhibitor rhodium(III) complex 6 (RHD6). The protein level of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. Relative expression levels of GATA6, TFF1, and TFF2 in (F) MKN45, (G) MKN28, and (H) AGS gastric cancer cells treated with dimethyl sulfoxide (DMSO) or 2.5 μm of RHD6. (*** p < 0.001; ** p < 0.01; * p < 0.05). Each bar represents mean ± standard deviation (SD) of duplicate experiments. n.s.: not significant.