Table 3.
Primer sequences used in this study.
| Sequence 5′–3′ | |
|---|---|
| RT Primer | |
| STAT3 forward | ACTTTCACTTGGGTGGAGAAGGACAT |
| STAT3 reverse | CTGCTGCTTTGTGTATGGTTCCA |
| GAPDH forward | CCCCTTCATTGACCTCAACTACAT |
| GAPDH reverse | CGCTCCTGGAAGATGGTGA |
| GATA6 forward | AGCCGGCCCCTCATCAAGCCGCAGAA |
| GATA6 reverse | AGTTGGCACAGGACAATCCAAGCC |
| TFF1 forward | CACCATGGAGAACAAGGTGA |
| TFF1 reverse | TGACACCAGGAAAACCACAA |
| TFF2 forward | ATGGATGCTGTTTCGACTCC |
| TFF2 reverse | AGAAGCAGCACTTCCGAGAG |
| ChIP-PCR | |
| GATA6 forward | CGATCACGGAAAGACACCTT |
| GATA6 reverse | CCAATGACCGACGAAAGATT |
| ACTB forward | TGCGTGACATTAAGGAGAAG |
| ACTB reverse | GCTCGTAGCTCTTCTCCA |
| Pyrosequencing | |
| GATA6 forward | GGGTGGGGGAGATTTGTAAG |
| GATA6 reverse | AGCTGGACATCACCTCCCACAACGAAACCTT |
| CTCCCTTATACCATATTTCTTCC | |
| GATA6 sequencing | GAGATTTAAATTTAAAGAAAATTAT |
| UB03 biotin primer | AGCTGGACATCACCTCCCACAACG |
RT: reverse transcription; STAT3: signal transducer and activator of transcription 3; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; TFF: trefoil factor; ChIP-PCR: chromatin immunoprecipitation-PCR; ACTB: β-actin.