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. 2004 Aug 16;114(4):560–568. doi: 10.1172/JCI22206

Figure 1.

Figure 1

MIC expression in prostate cancer cell lines and susceptibility of prostate cancer cells to NK cell activation in an NKG2D-dependent fashion. (A) Flow cytometry analysis of MIC expression in PrECs and the prostate cancer cell lines M12, PC-3, and LnCaP. White profiles represent staining with control mouse IgG (mIgG); black profiles represent staining with the BAMO1 mAb against MIC (MIC); and gray profiles represent staining with the W6/32 mAb against MHC I (MHC I). (B) Cytotoxicity of NK cells isolated from a healthy individual against M12 target cells at the indicated effector/target (E/T) ratios. NK cell cytotoxicity was inhibited by masking of NKG2D on NK cells or of MIC on M12 cells with 10 μg/ml of antibody M585 (+ anti-NKG2D) (31) or BAMO1 (+ anti-MIC) (30), respectively, but was not affected by control mouse IgG (+ IgG). Data shown are mean ± SD of triplicates. Results shown are representative of three independent experiments.