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. 2016 Sep 20;17(9):1484. doi: 10.3390/ijms17091484

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of concentrations of TSH (0, 0.1, 1, 10, 100 mIU/mL) in HUVECs over 24 h. (A) The bands depict representative findings of OPN, integrin α_vβ₃, and VCAM-1 protein expression levels in HUVECs stimulated by different concentration of TSH; (B) The bar graphs showed the results of the semiquantitative measurements of OPN, integrin α_vβ₃, and VCAM-1, respectively; (C) The bar graphs showed the results of the quantitative analysis of OPN, integrin α_vβ₃, and VCAM-1 from real-time PCR. The OPN, integrin α_vβ₃, and VCAM-1 mRNA levels were expressed as ratios relative to GAPDH. The results are shown as the means ± SEM of three independent experiments. * p < 0.05 versus control; ** p < 0.01 versus control.