Figure 5.

The effects of 10 mIU/mL TSH for 0, 2, 6, 12, 24, and 48 h in HUVECs. (A) The bands depicted representative protein expression level findings for OPN, integrin α_vβ₃, and VCAM-1 in HUVECs stimulated by different durations of TSH; (B) The bar graphs show the results of the semiquantitative measurements of OPN, integrin α_vβ₃, and VCAM-1; (C) The bar graphs show the results of the quantitative analysis of OPN, integrin α_vβ₃, and VCAM-1 from real-time PCR. The OPN, integrin α_vβ₃, and VCAM-1 mRNA levels are expressed as ratios relative to GAPDH. The results are shown as the means ± SEM of three independent experiments. * p < 0.05 versus control; ** p < 0.01 versus control.