Figure 2.

BL-038 induces cell apoptosis in chondrosarcoma cells. (A–D) The JJ012 and SW1353 chondrosarcoma cells were incubated with indicated conditions of BL-038 for 24 h, the cells were stained by annexin V/PI and percentage of apoptotic cells were analyzed by flow cytometric analysis; (E,F) cells were treated as described in (A), the cells were stained by propidium iodide (PI) and the apoptotic cells were assessed by flow cytometric analysis; (G,H) cells were treated with vehicle or BL-038 for 24 h. The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) positive cells were examined by flow cytometry. Results are expressed as the mean ± SEM. * p < 0.05 compared with controls.