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. 2016 Sep 8;17(9):1504. doi: 10.3390/ijms17091504

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Knockdown of Six2 gene accelerates cell apoptosis while LiCl treatment of low-concentration inhibits cell apoptosis in mK3 cells. (A) mK3 cells were transfected with negative shRNA control and Six2-shRNA for 36 h and treated with LiCl of increasing dosages. The apoptosis was detected by FCM; (B) Statistical analysis of cell apoptosis and histogram was drawn in GraphPad Prism 5; (C) The efficiency of knockdown Six2 at mRNA and protein level, compared with internal control 18S and β-tubulin, respectively. Values were presented as mean ± SEM (n = 3), *** p < 0.001 relative to control.