Table 4.
Spontaneously-formed TH1- and TH2-type cytokines in culture supernatants of splenocytes harvested from C57BL6 pups exposed in utero to PFOS during GD 1–17.
| Sex | PFOS | IL-2 | IFNγ | IL-4 | IL-10 |
|---|---|---|---|---|---|
| 4 weeks of age | |||||
| Male | 0.0 | 54.02 ± 4.20 | 24.67 ± 2.53 | 11.87 ± 2.02 | 53.21 ± 4.32 |
| 0.1 | 51.89 ± 5.21 | 22.38 ± 2.05 | 13.95 ± 1.35 | 57.75 ± 3.97 | |
| 1.0 | 47.08 ± 3.14 | 20.03 ± 1.70 | 15.81 ± 1.92 | 54.68 ± 4.21 | |
| 5.0 | 38.49 ± 3.68 a | 19.63 ± 1.53 | 17.78 ± 1.23 a | 60.61 ± 3.64 | |
| Female | 0.0 | 49.65 ± 4.01 | 22.07 ± 2.28 | 13.55 ± 1.85 | 49.45 ± 4.31 |
| 0.1 | 46.50 ± 3.07 | 24.90 ± 2.14 | 15.61 ± 1.09 | 53.96 ± 5.53 | |
| 1.0 | 41.45 ± 3.98 | 20.47 ± 1.79 | 17.59 ± 1.37 | 56.07 ± 4.78 | |
| 5.0 | 39.33 ± 2.99 | 18.03 ± 2.30 | 19.46 ± 1.93 a | 62.40 ± 4.99 | |
| 8 weeks of age | |||||
| Male | 0.0 | 34.35 ± 3.92 | 26.87 ± 2.99 | 15.32 ± 1.88 | 39.42 ± 4.61 |
| 0.1 | 31.76 ± 4.11 | 25.63 ± 3.74 | 16.71 ± 2.54 | 40.65 ± 5.62 | |
| 1.0 | 38.22 ± 3.83 | 23.68 ± 4.20 | 19.50 ± 1.93 | 42.98 ± 3.71 | |
| 5.0 | 32.42 ± 5.80 | 24.85 ± 3.52 | 21.79 ± 2.06 a | 46.93 ± 4.98 | |
| Female | 0.0 | 36.16 ± 5.84 | 25.40 ± 3.58 | 19.78 ± 3.20 | 36.19 ± 3.36 |
| 0.1 | 35.68 ± 3.70 | 24.39 ± 4.24 | 20.04 ± 2.78 | 38.47 ± 5.44 | |
| 1.0 | 37.27 ± 4.48 | 23.88 ± 3.82 | 21.72 ± 3.43 | 42.80 ± 3.28 | |
| 5.0 | 34.89 ± 4.53 | 21.92 ± 4.03 | 25.69 ± 2.56 | 41.34 ± 4.60 | |
Data are presented as the mean (±SE) of ELISA results. All values in pg/mL. PFOS doses in terms of mg/kg/day for dams. a Significantly different from respective sex/age control (p ≤ 0.05) by one-way ANOVA test. Data were log transformed as required for statistical analysis. n = 12 in each group.