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High sensitivity of dPCR-based chimerism detection. In the shown example, an artificial dilution of 0.01% MNCs positive for the marker DP114-I/DP131-I was generated in DP114-I-/DP131-I-negative MNCs. Six hundred nanograms of gDNA were subjected to duplex-dPCR using assays: (a) DP114-I (FAM, channel 1) vs. SRY (HEX, channel 2); and (b) DP131-I (FAM, channel 1) vs. Ref (HEX, channel 2).
