Table 1.
Pimers used for the amplification of banana Trx genes full cDNA length, construction of eGFP and pFL61 vectors and qPCR.
| Name | Nucleotide Sequence (5’-3’) |
|---|---|
| Trx-For1 | CGACTTCGCCGCCACNTGGTGYGGNC |
| Trx-For2 | CGTGGAGTTCGTCAAGATCGAYGTNGAYGA |
| MaTrx6-5RACE1 | CGGAGTACTGGGCGGCCATC |
| MaTrx6-5RACE2 | CTCCACGAAGCGGCACGGC |
| MaTrx9-5RACE1 | TGTTCCTCGTGGTTTAGTTG |
| MaTrx9-5RACE2 | ATCCTCAGCCGACTTGATA |
| MaTrx12-5RACE1 | GTTGCTGAGATGTCCCACGA |
| MaTrx12-5RACE2 | GCCACAACTATCCTGCCATC |
| eGFP-MaTrx6-Sal I-F | AGTCGACATGGGTGGTTTCTTCTCCAGCC |
| eGFP-MaTrx6-Sal I-R | AGTCGACGGTGTTGATCTGCTCTTGGATCC |
| eGFP-MaTrx9-Mlu I-F | CGACGCGTATGGCGGATGCTTTGGCTC |
| eGFP-MaTrx9-Mlu I-R | CGACGCGTTCTACCGGCTGCGGC |
| eGFP-MaTrx12-Sal I-F | AGTCGACATGGGAAACTGCATAAGAAAGAATGTGAG |
| eGFP-MaTrx12-Sal I-R | AGTCGACTCGTAGCGGGAGAGTAGCATC |
| MaTrx6-F | AGGAGGTGGGCAGAATCGT |
| MaTrx6-R | TGGTGGCGGTAATACAGACAG |
| MaTrx9-F | TCACCTACACAAGAACGCCT |
| MaTrx9-R | CCACATACATAACCAATAAGCAG |
| MaTrx12-F | TCGTGGGACATCTCAGCAAC |
| MaTrx12-R | CTTTCTTCATCGTAGCGGGA |
| Actin-F | TGGTATGGAAGCCGCTGGTA |
| Actin-R | TCTGCTGGAATGTGCTGAGG |
| pFL61-MaTrx6-Not I-F | TTGCGGCCGCATGGGTGGTTTCTTCTCCAGCC |
| pFL61-MaTrx6-Not I-R | TTGCGGCCGCTTAGGTGTTGATCTGCTCCTGGATCC |
| pFL61-MaTrx9-Not I-F | TTGCGGCCGCATGGCGGATGCTTTGGCTC |
| pFL61-MaTrx9-Not I-R | TTGCGGCCGCTCATCTACCGGCTGCGGCAAAAG |
| pFL61-MaTrx12-Not I-F | TTGCGGCCGCATGGGAAACTGCATAAGAAAG |
| pFL61-MaTrx12-Not I-R | TTGCGGCCGCTCATCGTAGCGGGAGAGTAGC |
| FLDR | CTATTATTTTAGCGTAAAGGATGG |
| FLGA | CTCTTTTTTACAGATCATCAAGG |