Figure 2.

The combination of MSCs and an EE induces astrocyte activation. (A–N) Two and eight weeks after an EE and MSC transplantation, the amounts of GFAP⁺ cells (green color) and CS-56⁺ cells (red color) were evaluated using the MetaMorph Imaging System. (A) The density of striatal GFAP⁺ cells (%) was significantly higher in the MSC-EE mice than in both the PBS-CON and PBS-EE groups two weeks after treatment (* p < 0.05 and ** p < 0.01, respectively; n = 5 each); (B) The CS-56⁺ cell density did not differ between groups; (C–N) Two weeks after an EE and MSC transplantation, representative images are shown. Scale bars = 100 μm. Values represent mean + SEM.