Table 1.
List of candidate reference genes used in the study. The details concerning the primer sequences, amplicon length and Tm, PCR efficiency and regression coefficient are given.
| Name | Sequence (5′→3′) | Amplicon Length (bp) | Amplicon Tm (°C) | PCR Efficiency (%) | Regression Coefficient (R2) |
|---|---|---|---|---|---|
| ActinFwd | TTGCTGGTCGTGATCTTACTG | 148 | 83 | 90.8 | 0.993 |
| ActinRev | GTCTCCATCTCCTGCTCAAAG | ||||
| eTIF4EFwd | AGTGGGAAGATCCTGAATGTGC | 150 | 81 | 95.1 | 0.997 |
| eTIF4ERev | TTGCGACCACACCACAAATC | ||||
| EF2Fwd | ACGCAACAGCTATCAGGAAC | 113 | 80.3 | 92.3 | 0.998 |
| EF2Rev | TGCAAAGACACGACCAAAGG | ||||
| GAPDHFwd | AATCGCAACCCAAACTCTGC | 123 | 81.1 | 99.4 | 0.995 |
| GAPDHRev | AGTGGCCGTTGCTTTAATGG | ||||
| CycloFwd | ACAACATGTCGAACCCCAAG | 106 | 81.4 | 93.8 | 0.998 |
| CycloRev | TCAGCGGTTTTTGGCGTAAC | ||||
| RANFwd | TTTGGAGACTTCAGCACTGG | 129 | 81.8 | 97.8 | 0.998 |
| RANRev | GCAGGGTTACCATTTCCTTG | ||||
| F-boxFwd | TATCGGCGGAGAGATTTGAG | 77 | 78.4 | 99.5 | 0.975 |
| F-boxRev | TAAGCCCTTCCCTTGATTCC | ||||
| ClathrinFwd | TGTCAGTTTTGTGCCACCAG | 139 | 80.3 | 98.3 | 0.998 |
| ClathrinRev | TCCATGCGTGTTCTACCAAG | ||||
| Histone3Fwd | TGAAGAAGCCTCATCGGTTC | 127 | 82.9 | 96.1 | 0.998 |
| Histone3Rev | TCTTGAGCGATTTCCCTGAC | ||||
| TIP41Fwd | TGAACAGTGGGGAGAAAAGC | 144 | 80.3 | 100.2 | 0.989 |
| TIP41Rev | GCTTCCTGTTTCCATCCAAG | ||||
| TubulinFwd | ATAACTGTACTGGGCTTCAAGG | 110 | 84 | 97.5 | 0.999 |
| TubulinRev | CCTGTGGAGATGGGTAAACTG | ||||
| CDPKFwd | GGTGGCTTTGCTTCTCTTTG | 86 | 78.7 | 97 | 0.986 |
| CDPKRev | GTCAAACCCCTTTTCACACC | ||||
| TA1Fwd | TTCGAGAAGTTCCCTCCAAC | 118 | 81.5 | 92.8 | 0.999 |
| TA1Rev | AGCCATATCCACAGCATTCC | ||||
| TA2Fwd | CTAGCAACCCAGCGATTTTC | 126 | 81.3 | 97 | 0.998 |
| TA2Rev | ACCACAAGCTCCCAATATGC | ||||
| DHS1Fwd | TGAGACTTTCCCTCCGATTG | 144 | 84.6 | 96.8 | 0.998 |
| DHS1Rev | TCAGCACAATCTCCACCTTG | ||||
| DHS2Fwd | TATCAAGGCTGTTCGTGGAG | 129 | 81.8 | 103 | 0.997 |
| DHS2Rev | AGGTGCTTTGATGGTGTTCC |