Table 2.
Ranking of the 12 candidate reference genes according to the different methods used.
| GeNormPLUS | NormFinder | BestKeeper | Comparative delta-Ct | RefFinder | |||||
|---|---|---|---|---|---|---|---|---|---|
| Gene | Stability Coeff. | Gene | Stability Coeff. | Gene | Stability Coeff. | Gene | Stability Coeff. | Gene | Stability Coeff. |
| Histone3 | 1.22 | Histone3 | 0.812 | Histone3 | 1.58 | Histone3 | 1.678 | Histone3 | 12 |
| EF2 | 1.135 | EF2 | 0.743 | RAN | 1.085 | EF2 | 1.549 | EF2 | 10.462 |
| Actin | 1.061 | Tubulin | 0.579 | Tubulin | 1.059 | Actin | 1.343 | Actin | 8.409 |
| Cyclo | 1.028 | Cyclo | 0.523 | EF2 | 0.926 | GAPDH | 1.335 | Cyclo | 6.701 |
| GAPDH | 0.959 | Actin | 0.518 | Clathrin | 0.926 | Clathrin | 1.33 | Clathrin | 6.447 |
| eTIF4E | 0.857 | Clathrin | 0.504 | eTIF4E | 0.888 | Cyclo | 1.319 | Tubulin | 5.948 |
| TIP41 | 0.798 | TIP41 | 0.502 | F-box | 0.885 | RAN | 1.302 | GAPDH | 5.635 |
| Tubulin | 0.703 | GAPDH | 0.462 | Actin | 0.87 | Tubulin | 1.299 | RAN | 4.461 |
| CDPK | 0.599 | RAN | 0.453 | Cyclo | 0.718 | TIP41 | 1.241 | eTIF4E | 3.742 |
| RAN | 0.503 | Fbox | 0.452 | CDPK | 0.699 | F-box | 1.234 | TIP41 | 2.913 |
| Clathrin | 0.47 | CDPK | 0.272 | GAPDH | 0.681 | eTIF4E | 1.155 | F-box | 2.913 |
| F-box | 0.45 | eTIF4E | 0.257 | TIP41 | 0.601 | CDPK | 1.091 | CDPK | 1.861 |