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. 2016 Sep 19;17(9):1560. doi: 10.3390/ijms17091560

Figure 4.

Figure 4

Influence of 2a on Topo I activity. The rate at which relaxed plasmid DNA was converted to supercoiled molecule was monitored over a time course of 30 min. Lane 1—supercoiled plasmid, SC; lane 2—plasmid with Topo I after 30 min of incubation time; lane 3–7—plasmid with Topo I and in the presence of compound 2a at the concentration of 15 μM after following incubation times: 1; 5; 10; 15; 30 min, respectively; lane 8–12—plasmid with Topo I and 9AA at the concentration of 100 µM after following incubation times: 1; 5; 10; 15; 30 min, respectively (positive control).