Figure 1.

Nested experimental design with three levels. Each variance level had multiple replicates—to assess natural variation, 19 accessions of Arabidopsis thaliana (A. thaliana) were grown. Three independent biological experiments were performed to estimate non-biological variance derived from the experimental batch. To assess individual variability, four plants were harvested in each biological experiment for each accession. Single-plant root extracts were subjected to liquid chromatography-coupled mass spectrometry (LC/MS) and gas chromatography-coupled mass spectrometry (GC/MS) analysis.