Figure 8.

Y-27632-induced Ca²⁺ sensitization is not involved in synergy between β₂-adrenoceptor agonists and muscarinic receptor antagonists. In each panel, percent inhibition of tension is represented by white columns, and F340/F380 is represented by black columns. (a) Left, percent inhibition of tension and F340/F380 with Y-27632 (1 µM); Center, sum of percent inhibition of tension and F340/F380 with Y-27632 (1 µM) and glycopyrronium (3 nM); Right, percent inhibition of tension and F340/F380 with combination of Y-27632 (1 µM) and glycopyrronium (3 nM); (b) Left, sum of percent inhibition of tension and F340/F380 with procaterol (0.3 nM) and glycopyrronium (3 nM); Center, percent inhibition of tension and F340/F380 with combination of procaterol (0.3 nM) and glycopyrronium (3 nM); Right, percent inhibition of tension and F340/F380 with combination of procaterol (0.3 nM) and glycopyrronium (3 nM) in the presence of Y-27632 (1 µM). GB, glycopyrronium bromide; PRO, procaterol; MCh, methacholine. *** p < 0.001; ** p < 0.01; * p < 0.05.