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. 2016 Sep 26;9:452. doi: 10.1186/s13104-016-2258-1

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Cytofluorometric detection of B-10665 (1 nM) in detached HEK 293a cells stably expressing human myc-tagged B₂Rs and competition of binding by the alternate antagonist icatibant. a Sample histograms for infrared fluorescence. Detached cells were treated for 30 min with B-10665; icatibant was applied 15 min before B-10665 when utilized. b Concentration-effect relationship for cell labeling with B-10665 and competition with icatibant in replicated experiments