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. 2016 Sep 26;16:208. doi: 10.1186/s12870-016-0898-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The ssi2-2 mutant harbors a new allele of SSI2. a Physical mapping of ssi2-2. Rough mapping showed that the ssi2-2 gene is located between markers NGA168 and CER461445 on chromosome 2 of Arabidopsis. Fine mapping revealed the gene between the primers of CAPS5 and CH2.1814, a range of 83.8 kb. The numbers below the molecular markers indicate the recombinant events detected between the ssi2-2 locus and the marker. b Structure of the ssi2-2 gene, AT2G43710. AT2G43710 encodes stearoyl desaturase and has three exons and two introns; sequence analysis revealed two point mutations in the third exon of the gene, namely G1952A and G2118A, resulting in changes in amino acids 257 and 312