Fig. 3.

Complementation of the ssi2-2 mutant. a Phenotypes of the transgenic complementation lines. Constructs harboring each single mutation did not rescue the dwarf phenotype of ssi2-2. Plants were photographed 3 weeks after germination. b The bacterial population of Pst DC3000 in the leaves of wild-type Col-0, ssi2-2 mutant and transgenic complementation lines. The seedlings were cultured for 4 weeks. Three leaves were harvested from infected leaves and then weighed, together homogenized in 10 mM MgCl₂, diluted 10⁵-or 10⁶-fold, and plated on King’ s B medium. The bacterial population was determined from three replicates at each time point by counting colony-forming units (cfu). The “0 day” point represents 2 h after bacterial inoculation. The data are shown as means ± SD from three biological replicates. The experiments were repeated three times. Significance was determined by Student’s t test. * P < 0.05