Fig. 4.

Allele structure and phenotypic differences in ssi2-1, ssi2-2 and ssi2-3. a The C-to-T mutation in ssi2-1 at nucleotide position 1543 changes the leucine (L) at amino acid position 146 to a phenylalanine (F) [10]. The TAIR database shows the T-DNA insertion in the first intron of SSI2 in ssi2-3 (SALK_039852). b The phenotype of the Col-0 wild-type and different ssi2 lines grown in a culture chamber at 22 °C for 4 weeks. c Biomass analyses of the indicated lines. The wild-type and mutants were cultured for 4 weeks at 22 °C. Data were analyzed by one-way ANOVA, and mean separation was calculated by multiple comparison Tukey’s test (P < 0.05). Nine plants were used as biological replicates for each. d ROS quantitation in the leaves of wt (Col-0), ssi2-1, ssi2-2 and ssi2-3 plants. The leaves directly treated with 10 mM H₂DCF-DA dissolved in PBS at 37 °C for 30 min. The epidermis was isolated and the fluorescence intensity was monitored. Quantification of ROS was performed using an ImagePro Plus analysis package