Fig. 5.

Marker gene expression, SA content and bacterial growth in Col-0 and different ssi2 mutant lines. Col-0 and different ssi2 mutant lines were treated with water and 50 μM salicylic acid (SA) or 50 μM jasmonic acid (JA), and samples were taken 24 h after treatment. The expression levels of PR1 (a) and PDF1.2, VSP2 (b) are shown. The test performed by quantitative RT-PCR analysis. Transcript abundance of genes was normalized to that of the reference gene ACTIN2 (AT3G18780). The data are shown as means ± SD from three biological replicates. The experiments were repeated three times. ** P < 0.01. c SA levels in the leaves of wt (Col-0), ssi2-1, ssi2-2 and ssi2-3 plants. d Growth of Pst DC3000 in the leaves of wild-type and different ssi2 mutant lines cultured for 4 weeks. The 0 d time point represents 2 h after bacterial inoculation. The data are shown as means ± SD from three biological replicates. The experiments were repeated three times. Significance was determined by Student’s t test. *Indicates significant differences at P < 0.05, ** Indicates significant differences at P < 0.01