Table 1.
Structure-based sequence alignment of CDKs for the chemical-genetic approach
| Kinase | ß-sheets | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ß2 | ß3 | ß4 | ß5 | ||||||||||||
| Cdk1 (H.s.) | V | V | Y | V | A | M | K | K | I | V | S | Y | L | I | F 80 |
| Cdk2 (H.s.) | V | V | Y | V | A | L | K | K | I | V | K | Y | L | V | F 80 |
| Cdk3 (H.s.) | V | V | Y | V | A | L | K | K | I | V | R | Y | L | V | F 80 |
| Cdc2 (S.p.) | V | V | Y | V | A | M | K | K | C | V | R | Y | L | V | F 84 |
| CDC28 (S.c.) | V | V | Y | V | A | L | K | K | I | V | R | Y | L | V | F 88 |
| CDKA;1 (A.t.) | V | V | Y | I | A | L | K | K | I | V | K | Y | L | V | F 80 |
Homo sapiens (H.s.), Schizosaccharomyces pombe (S.p.), Saccharomyces cerevisiae (S.c.), Arabisopsis thaliana (A.t.). Bold letters mark residues contacting ATP in the active site. Numbers indicate the positions of the respective residues in the protein. The “gatekeeper” positions are numbered