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. 2016 Sep 23;4(18):e12896. doi: 10.14814/phy2.12896

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Control for potential effects of different OGA inhibitors, rat strains, and O‐GlcNAc antibodies on protein O‐GlcNAcylation. Muscle cell lysates of soleus from Wistar (W) containing glucosamine was compared to lysates containing the OGA inhibitor PUGNAc (A), revealing identical O‐GlcNAcylation patterns by the two methods. Furthermore, muscle cell lysates of soleus from both Wistar and Sprague Dawley (SD) rats were analyzed for O‐GlcNAcylation pattern using the CTD110.6 antibody (B), showing no differences between the strains. Finally, parallel analysis of the same samples as in B with the RL2 antibody (C) showed a slightly different O‐GlcNAcylation pattern compared to B, as expected from the literature. However, neither the RL2 antibody revealed any differences in O‐GlcNAcylation pattern between the rat strains (n = 3).