Figure 2.

End‐point PCR experiments investigating UT‐A6 expression in human colon. (A) Using cDNA derived from pooled total RNA adult colon samples (N = 5), strong signals were detected for actin (514 bp) and AQP3 (286 bp). In contrast, weaker signals were detect for UT‐A6 using two separate sets of primers designed against the UT‐A6‐specific exon 11 (128 and 136 bp signals). These signals were confirmed as UT‐A6 by sequencing of the PCR products. (B) Using cDNA derived from total RNA adult and fetal colon samples (each N = 1), a weak UT‐A6 signal was again detected in adult colon, but not in fetal colon. Key: RT = reverse transcriptase; + = RT present; − = RT absent.