Figure 2.

The ΔMosec4 mutants showed defects in vegetative growth and conidial morphogenesis. Results shown for Ku80, ΔMosec4 (ΔMosec4-11), and ΔMosec4-Com (the ΔMosec4-11 complemented strain) are from three independent experiments with standard deviations indicated. Similar hyphal growth and conidiation results were observed for independent mutant ΔMosec4-46. (A) Diameter of hyphal growth at 10 days after incubation on starch yeast medium (SYM), rice bran medium (RDC), and complete medium (CM) agar plates at room temperature. See Figure S2 for more details. (B) Numbers of conidia harvested from a 9 cm rice bran plate after incubation for 7 days at room temperature. (C) Conidial development was observed microscopically 24 h after induction of conidiation. Bar = 20 μm. (D) Differential interference contrast (DIC) microscopy of conidial morphology. Bar = 20 μm. The letters indicate statistically significant differences (p < 0.01).