Figure 8.

Localization of cytoplasmic effector to BICs was partially disrupted in the ΔMosec4 mutant. The apoplastic effector fusion BAS4-GFP and cytoplasmic effector fusion PWL2-mCherry-NLS, engineered with a C-terminal nuclear localization signal (NLS) to concentrate translocated effector protein in the rice nucleus, were introduced into GUY11, the Ku80 and the ΔMosec4 mutant. Transformants were inoculated into the leaf sheath of susceptible rice YT16. White arrows indicate BICs, white arrowhead indicates the BIC-like structure, and white stars indicate PWL2-mCherry-NLS fluorescence in rice nuclei. Bars = 20 μm.